11081 Tram1l1 Status: ES cell colonies screened / QC no positives
| Gene | Tram1l1 |
| Chromosome | 3 |
| Deletion Start | 124,321,177 |
| Deletion End | 124,322,312 |
| Deletion Size | 1,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,321,362 | 124,321,379 | TCGTTGTCCCAGCGGAAG |
| TUP | 124,321,386 | 124,321,407 | CCTCCGGGTCCAGGACCCTGTA |
| TUR | 124,321,436 | 124,321,457 | CCACCAGCATGTAGAAGAACAC |
| TDF | 124,321,897 | 124,321,919 | CAGCCTGCTTTACTTTGGTGATG |
| TDP | 124,321,920 | 124,321,946 | AGCGGTACCAGAAAGGGTTGTCTCTGT |
| TDR | 124,321,955 | 124,321,978 | CACGAGTCTCCCGGATATAAACAC |
PCR Primers
| Primer | Sequence |
| SU | GACAGTGGCACCAGATTTCC |
| SD | GCGATAAAGAACACTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 392 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
