11085   Hemt1     Status: Cancelled

GeneHemt1
Chromosome15
Deletion Start74,820,570
Deletion End74,824,388
Deletion Size3,819
Genome Build38
MGI IDMGI:1341831
NCBI Gene ID15202
BAC ID
BAC Library
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,821,46574,821,484TGGACCCAGATACACCACTC
TUP74,816,25174,816,276TCTGGTAGCTCCTGGAACTGAGAGCC
TUR74,821,54174,821,563TGCCATTAGGCACACTACTCAAG
TDF74,823,96674,823,990GGTAATTCTCCTGATGGCTTCTTTG
TDP74,823,99274,824,018AGGTGTGAACATTCGGCTGCTCTATGT
TDR74,824,02074,824,039TCCTTGGTGCAGTCCTTGAG

PCR Primers

PrimerSequence
SUGATGTGGACTATGTCCATGG
SDTTCCCTTCTCCAATGACCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR74 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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