11086   Ndufb10     Status: ES cell colonies screened / QC no positives

GeneNdufb10
Chromosome17
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1915592
NCBI Gene ID68342
BAC ID60M20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTGGAATCCGGGAGGTTGTG
TUPTGCATGCCTTTAATCCCAGCACTGG
TURACCAGGCTGGCCTCAAAC
TDFCGGCCAGTTTGGGACTACAG
TDPACCCTTCGCCTCAAGCTGCTTCA
TDRGCGGAAGAGAACACCTTGAAC

PCR Primers

PrimerSequence
SUTACTCTTCCAAGCCATGTTC
SDACCAGAGTCTCTGTTCAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image