11091 Vps16 Status: Cancelled
| Gene | Vps16 |
| Chromosome | 2 |
| Deletion Start | 130,424,379 |
| Deletion End | 130,443,686 |
| Deletion Size | 19,308 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,424,595 | 130,424,617 | GTCTGCCACTTTACGCACTTTAG |
| TUP | 130,424,622 | 130,424,648 | AGCAATCTGGTCCTTCCTTTGCGCCAA |
| TUR | 130,424,649 | 130,424,667 | GCTTCTCAGCTTGCCTCAG |
| TDF | 130,443,544 | 130,443,565 | TGAGCACCGGAATGAAACAGAG |
| TDP | 130,443,577 | 130,443,600 | TTTGTCCCACTGCACTGGAGCCAC |
| TDR | 130,443,617 | 130,443,635 | TGGCCCGCTGAATCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCATGGCTCCCACTTTCGG |
| SD | CCCGGGTACCGTGGTGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 358 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
