11095 Gimap7 Status: Cancelled
| Gene | Gimap7 |
| Chromosome | 6 |
| Deletion Start | 48,723,485 |
| Deletion End | 48,724,364 |
| Deletion Size | 880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,723,601 | 48,723,619 | CCATGCTGTCACCCAGAAC |
| TUP | 48,723,620 | 48,723,642 | TGTCAGAGTGACTCCCGCAGGTG |
| TUR | 48,723,647 | 48,723,669 | TCAACAACCAGGAGGTCTCTTTC |
| TDF | 48,724,009 | 48,724,027 | GGAAACGCAGGTGCAGGAG |
| TDP | 48,724,037 | 48,724,066 | CTTGTAGAAACCCTGGTGCAGAACAATGGT |
| TDR | 48,724,068 | 48,724,089 | ACGGGATGGGAGAAGTAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGATTGTTAACGCTTC |
| SD | AGTGTGATCCATCCAGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
