11096 Cldn23 Status: ES cell colonies screened / QC no positives
| Gene | Cldn23 |
| Chromosome | 8 |
| Deletion Start | 35,826,329 |
| Deletion End | 35,825,422 |
| Deletion Size | 908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,826,219 | 35,826,239 | CTGGTGAAGGGCTTTCTGGAC |
| TUP | 35,826,193 | 35,826,215 | CCAGTGGACGTGGTGCTGTACCA |
| TUR | 35,826,104 | 35,826,123 | CAGGCTGGGTCTGGAAGTAG |
| TDF | 35,825,601 | 35,825,620 | GGGACACCAGCAAGCTCAAG |
| TDP | 35,825,577 | 35,825,599 | TTGGATTCCCGATGCCACGACCG |
| TDR | 35,825,533 | 35,825,553 | TCTCCCTCAAGCACATCCATC |
PCR Primers
| Primer | Sequence |
| SU | GTTTGGCCTTGGGGGCCGCACC |
| SD | GTAACTTAGAATACTGTACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 402 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
