11098 Zadh2 Status: Cancelled
| Gene | Zadh2 |
| Chromosome | 18 |
| Deletion Start | 84,088,168 |
| Deletion End | 84,095,337 |
| Deletion Size | 7,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,088,473 | 84,088,491 | GGCGCACAGCCTGACTTTG |
| TUP | 84,088,500 | 84,088,522 | CAGGAAGTTGAACCCGCCGCCAT |
| TUR | 84,088,547 | 84,088,567 | TCGGGTATTCACACCAGCAAG |
| TDF | 84,094,985 | 84,095,004 | CCAAAGGGCGCTTGATAGTG |
| TDP | 84,095,006 | 84,095,033 | TTGGGTTTATCTCTGGCTACCAAAGCCC |
| TDR | 84,095,069 | 84,095,090 | GACTTCTTCAGGAGCTTGGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGGGATATCCGGGGCCTCTC |
| SD | GGTGAGCTCTCTGAAGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 501 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 425 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
