11099 Otud1 Status: ES cell colonies screened / QC no positives
| Gene | Otud1 |
| Chromosome | 2 |
| Deletion Start | 19,658,065 |
| Deletion End | 19,659,429 |
| Deletion Size | 1,365 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,658,452 | 19,658,469 | GCCTGGCTGCTGGAAGAG |
| TUP | 19,658,470 | 19,658,488 | CTGCTCCGGCCCGACGAGC |
| TUR | 19,658,521 | 19,658,541 | GCTCAGTCGGAAGTTCCTGTC |
| TDF | 19,659,192 | 19,659,216 | CGTGTCTACCATGATCCACTATCTG |
| TDP | 19,659,219 | 19,659,244 | CCCAGAGGATTCTCTACGGCCCAGTA |
| TDR | 19,659,252 | 19,659,271 | GTCCATTGCTGAGCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | CAGCTCCTCCAACCGTCCC |
| SD | CCGTGAGACATCAGCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 415 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 371 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
