11105 Imp3 Status: Cancelled
| Gene | Imp3 |
| Chromosome | 9 |
| Deletion Start | 56,937,491 |
| Deletion End | 56,938,063 |
| Deletion Size | 573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,937,544 | 56,937,566 | TGAAACAGGTGGACTTCCTAAAC |
| TUP | 56,937,567 | 56,937,588 | TGGGAAGTCACCGACCACAACC |
| TUR | 56,937,594 | 56,937,610 | CGCAGCACACGCAGTTC |
| TDF | 56,937,886 | 56,937,905 | CTGTGGCTTTTGTGGAGCAG |
| TDP | 56,937,925 | 56,937,950 | CAGACGTGGTCACTGATCCCGCCTTT |
| TDR | 56,937,992 | 56,938,011 | GCCGCTTGATCTTGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | AACACTGGACTAGACTAGTTG |
| SD | ACTCTCCATCCAGACGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
