| Gene | Naaa |
| Chromosome | 5 |
| Deletion Start | 92,278,129 |
| Deletion End | 92,259,535 |
| Deletion Size | 18,595 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,277,630 | 92,277,650 | AGGAAACTCCGCATCGTGGAC |
| TUP | 92,277,606 | 92,277,629 | CAGCCTGGCTGGCCTTACATGGAG |
| TUR | 92,277,552 | 92,277,573 | AAGGGCTGAGATACACCCAATG |
| TDF | 92,259,735 | 92,259,756 | TCTGCCTCTTTGCATTCTTACC |
| TDP | 92,259,699 | 92,259,726 | TTATCCTGTAACCTTGTCCTGCATCCCA |
| TDR | 92,259,680 | 92,259,698 | GATGCTCTCCCGAATCTCC |
PCR Primers
| Primer | Sequence |
| SU | CGTATAGCACAAATCACCAGG |
| SD | GCGATAGCTAGCCTTGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11112B-G7 | VGB6 | C57BL/6NTac | |
| 11112B-D7 | VGB6 | C57BL/6NTac | |
