11114 Rnase13 Status: ES cell colonies screened / QC no positives
| Gene | Rnase13 |
| Chromosome | 14 |
| Deletion Start | 51,922,677 |
| Deletion End | 51,922,200 |
| Deletion Size | 478 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,922,639 | 51,922,656 | CTTGTCCTCCCGCTTGTC |
| TUP | 51,922,616 | 51,922,638 | TTCCGGCCAACTCTGGTGACAGG |
| TUR | 51,922,574 | 51,922,598 | TGTAAGGTTCGGAAGTTCTTGATGG |
| TDF | 51,922,364 | 51,922,383 | TGCACCCTCACTCAGAACTC |
| TDP | 51,922,337 | 51,922,359 | CCCGTCACAATCTGCACCCTGGT |
| TDR | 51,922,314 | 51,922,335 | AGCTGGTTGGTGCCTGTTGATG |
PCR Primers
| Primer | Sequence |
| SU | CTTGTGAATCTGTGCTGCAC |
| SD | GTTGCCATGGTGTGATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
