11128   Iqcf4     Status: ES cell colonies screened / QC no positives

GeneIqcf4
Chromosome9
Deletion Start106,570,865
Deletion End106,568,212
Deletion Size2,654
Genome Build38
MGI IDMGI:1914570
NCBI Gene ID67320
BAC ID179P21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,570,565106,570,587TCAGACCTTCTTCCACTCCAAAG
TUP106,570,542106,570,563TGGAACACACGTGGCCCTCGAA
TUR106,570,481106,570,502GACCACGGTAAGTCTGGGTAAC
TDF106,568,576106,568,598CTGCACTTGATGGCACAAGAAAC
TDP106,568,549106,568,575AAGGGCCTGCGTCCTTATTCAGTCGTG
TDR106,568,495106,568,512CGGGTGGCATAGCACAAC

PCR Primers

PrimerSequence
SUAACATTTCCCGTCTTTACTC
SDTCGAGATGCTTAAATGGCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev414 bp-++
TUFTUR107 bp++-
TDFTDR104 bp++-
NeoFwdSD369 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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