11131 Arsj Status: ES cell colonies screened / QC no positives
| Gene | Arsj |
| Chromosome | 3 |
| Deletion Start | 126,364,758 |
| Deletion End | 126,365,159 |
| Deletion Size | 402 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,364,825 | 126,364,842 | TGGGCCTGGTCTGGAAAG |
| TUP | 126,364,843 | 126,364,865 | TTCTTAGCGATGGGTGCCCTGGC |
| TUR | 126,364,893 | 126,364,912 | CCCAGCACAGGTAGCCATAG |
| TDF | 126,365,064 | 126,365,083 | GACGCCCACCCTTGATAAAC |
| TDP | 126,365,084 | 126,365,110 | TCGCTGCAGAAGGAGTTAAGCTAGAGA |
| TDR | 126,365,126 | 126,365,147 | CTCCTGGACGGTGTGCAAATAG |
PCR Primers
| Primer | Sequence |
| SU | GTGAAGAGGACATCTGCTCC |
| SD | AGGAAAGGGAGGAGATTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
