11138 B4galt4 Status: ES cell colonies screened / QC no positives
| Gene | B4galt4 |
| Chromosome | 16 |
| Deletion Start | 38,752,150 |
| Deletion End | 38,768,080 |
| Deletion Size | 15,952 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,752,559 | 38,752,579 | TGTGAGGCCCTTACTCCTTTC |
| TUP | 38,752,583 | 38,752,610 | CCATATCCTGACTCCAGGACTCATGGGC |
| TUR | 38,752,615 | 38,752,634 | GCGTCCTGTCTGTCAGGTTC |
| TDF | 38,767,566 | 38,767,586 | TGATGTTTAGCCAAGGACCTG |
| TDP | 38,767,588 | 38,767,609 | TCCGAGTGGGCAGCACATACCC |
| TDR | 38,767,615 | 38,767,639 | GAGGGACTACAACAACCACTGATAG |
PCR Primers
| Primer | Sequence |
| SU | TAGCATCTCACCGAGACCG |
| SD | ATCTGGAATCCACCACTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 472 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
