| Gene | Vsnl1 |
| Chromosome | 12 |
| Deletion Start | 11,386,973 |
| Deletion End | 11,386,800 |
| Deletion Size | 174 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,386,945 | 11,386,966 | AGAATAGCAAACTGGCCCCAGA |
| TUP | 11,386,919 | 11,386,942 | TCATGGAGGACCTGGTGAAGAGCA |
| TUR | 11,386,896 | 11,386,918 | AAGCTCATGCTCATTGAACTCAG |
| TDF | 11,386,872 | 11,386,892 | CAGTGGTACAAGGGCTTCCTC |
| TDP | 11,386,845 | 11,386,867 | ACTGTCCGAGCGGGAGGCTGAAC |
| TDR | 11,386,818 | 11,386,839 | CACATAGAGCTGCTGGAACTCC |
PCR Primers
| Primer | Sequence |
| SU | CAGGAACAATAGCTGTCTGG |
| SD | TCTTGTTCAGAAATTTGCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11139B-D2 | VGB6 | C57BL/6NTac | |
| 11139B-D7 | VGB6 | C57BL/6NTac | |
| 11139B-D9 | VGB6 | C57BL/6NTac | |
| 11139B-E4 | VGB6 | C57BL/6NTac | |
| 11139B-G9 | VGB6 | C57BL/6NTac | |
