11140 Tktl2 Status: Cancelled
| Gene | Tktl2 |
| Chromosome | 8 |
| Deletion Start | 66,511,795 |
| Deletion End | 66,513,909 |
| Deletion Size | 2,133 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,511,908 | 66,511,925 | TCCCACGTCGTGCTGTAG |
| TUP | 66,511,928 | 66,511,950 | TGGCAGAGATCATGGCTGTGCTC |
| TUR | 66,511,971 | 66,511,990 | GTTCTGGGTCTGCCTGTTTG |
| TDF | 66,513,417 | 66,513,437 | CAAACCTCTGGATGCTGTCAC |
| TDP | 66,513,441 | 66,513,467 | TATCCAAAGTGCCAAAGCCACAGGTGG |
| TDR | 66,513,497 | 66,513,516 | TTCACCAATGCCACCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | ATAATGTTGAGCCTGAAAGTG |
| SD | CTATCGACTCTTGCTAAAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 256 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
