11141 Rgag4 Status: ES cell colonies screened / QC positives
| Gene | Rgag4 |
| Chromosome | X |
| Deletion Start | 102,070,859 |
| Deletion End | 102,069,020 |
| Deletion Size | 1,840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,070,101 | 102,070,120 | GCAAGCTCAAGCAGGGAAAC |
| TUP | 102,070,078 | 102,070,100 | TGTACCCTGGGCAGCTATGCAGA |
| TUR | 102,070,032 | 102,070,053 | GCGACAGTCATCCCAAGACAAG |
| TDF | 102,069,242 | 102,069,262 | GCAGACAGAGGAGCCAGAATC |
| TDP | 102,069,216 | 102,069,237 | CCCACTTCTGGAGGGCCTTCCC |
| TDR | 102,069,190 | 102,069,213 | GGGCTGTAGAAGGATGAATTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTGCCTGCTCTCTCTC |
| SD | AAGATTGGGGACAACTAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 331 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 405 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
