| Gene | Pex5l |
| Chromosome | 3 |
| Deletion Start | 33,082,065 |
| Deletion End | 33,080,473 |
| Deletion Size | 1,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,081,864 | 33,081,883 | GAGGACCCAGAAGCCAAGAG |
| TUP | 33,081,834 | 33,081,861 | AGGAAGTCAGCTTGGCTCAAGCCAAACA |
| TUR | 33,081,739 | 33,081,761 | CAGGACAGAACAAATGGTCACAG |
| TDF | 33,081,158 | 33,081,179 | TTGCTGTAGGGAAGATGGGTAG |
| TDP | 33,081,133 | 33,081,155 | AGCACTCATTAGGGCCAGCCACA |
| TDR | 33,081,057 | 33,081,081 | TAGGTAGCTTATGAAGTTGGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACATAGTCTATATGTAAGAG |
| SD | TGACTCATTGCATTGAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 449 bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | 367 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11153A-D8 | VGB6 | C57BL/6NTac | |
| 11153A-F9 | VGB6 | C57BL/6NTac | |
