11159 Ccdc157 Status: Cancelled
| Gene | Ccdc157 |
| Chromosome | 11 |
| Deletion Start | 4,150,041 |
| Deletion End | 4,143,591 |
| Deletion Size | 6,451 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,149,861 | 4,149,881 | TCTAGCTTCTTACCACACCAC |
| TUP | 4,149,833 | 4,149,858 | CATGTACCTCATCCTTTGCCGCCTCC |
| TUR | 4,149,814 | 4,149,831 | TGGGAGACATGGGCAGTG |
| TDF | 4,143,936 | 4,143,960 | GGTTGCTGTTCCTTTCTTCCCTTAG |
| TDP | 4,143,909 | 4,143,931 | ACAGGCAGACGGCAGTCTCCTGG |
| TDR | 4,143,887 | 4,143,905 | GCCTGTGCTGCTTGTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGAGCTGCCTCTGACTG |
| SD | GCTCCTAGCCTAGCTCGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 546 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 387 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
