11166   Dub2a     Status: ES cell colonies screened / QC no positives

GeneDub2a
Chromosome7
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:3051372
NCBI Gene ID384701
BAC ID95K6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF103,252,243103,252,267CAAAACCAGACAGGAACTGATGAGG
TUP103,252,210103,252,236CACTGTGTGGCCTTTTTACCAGCTAGC
TUR103,252,154103,252,173GGAGCACACCATCCCCAAAG
TDF103,250,264103,250,285TTCACCTGCCCAGATCCATAGC
TDP103,250,220103,250,248TACTCCAGACAAGGTGAATCAACCCTGGC
TDR103,250,168103,250,187AGCTGCCCAGTGTTCACAAG

PCR Primers

PrimerSequence
SUCCCAAGCTTAAGACACCTAG
SDTACGCTGCTCAGATCCCATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR114 bp++-
TDFTDR118 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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