11168 Rpl36a Status: ES cell colonies screened / QC no positives
| Gene | Rpl36a |
| Chromosome | X |
| Deletion Start | 134,585,701 |
| Deletion End | 134,587,957 |
| Deletion Size | 2,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,586,022 | 134,586,039 | GAGGACTCTGCGCCATTG |
| TUP | 134,586,040 | 134,586,062 | TGGTCCGGTAACCGAGTGCTGTA |
| TUR | 134,586,110 | 134,586,131 | TTGGTGCTTCCCACATTTCTTG |
| TDF | 134,587,240 | 134,587,261 | AGCACAGGCTAACCTCACATTC |
| TDP | 134,587,263 | 134,587,285 | TGTGGCTGTACCTTGCCTCTGGG |
| TDR | 134,587,291 | 134,587,309 | GCTTGCTGCCACACACTTG |
PCR Primers
| Primer | Sequence |
| SU | TCCGCCAACAGGGATAAAGC |
| SD | GAAATACAAGTCTCTACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 417 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
