| Gene | Nat3 |
| Chromosome | 8 |
| Deletion Start | 67,547,474 |
| Deletion End | 67,548,420 |
| Deletion Size | 947 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,547,663 | 67,547,683 | GGAGGGTGGTGTCTTCAAGTC |
| TUP | 67,547,685 | 67,547,711 | ACCATCTTCTCTACTGGGCTCTGGCCA |
| TUR | 67,547,742 | 67,547,764 | GGCTGAAGGGACATAAACACATC |
| TDF | 67,548,053 | 67,548,076 | GAACCACGAACGATTGAAGATTTC |
| TDP | 67,548,095 | 67,548,124 | TACCAGGTATCTCGAACATCTGTGATGACA |
| TDR | 67,548,138 | 67,548,160 | TCCGTCTTTGGTATGTAGGGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCCTTCACCATAGCATTTG |
| SD | ATCAGCACTGTACACTTTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11170A-A4 | VGB6 | C57BL/6NTac | |
| 11170A-B4 | VGB6 | C57BL/6NTac | |
| 11170A-D3 | VGB6 | C57BL/6NTac | |
| 11170A-F6 | VGB6 | C57BL/6NTac | |
| 11170A-A12 | VGB6 | C57BL/6NTac | |
