11173 Kcnk12 Status: ES cell colonies screened / QC no positives
| Gene | Kcnk12 |
| Chromosome | 17 |
| Deletion Start | 87,797,451 |
| Deletion End | 87,797,052 |
| Deletion Size | 400 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,797,350 | 87,797,367 | TCGCACCTCAACGAGGAC |
| TUP | 87,797,330 | 87,797,349 | ACCGGCCGCTTCGTGCTGCT |
| TUR | 87,797,268 | 87,797,287 | GGCTTTCGAGCGCAGAGAAC |
| TDF | 87,797,174 | 87,797,189 | AGCTGCGCGCCTTCCT |
| TDP | 87,797,150 | 87,797,169 | CACTACGAGGCCGCGCTGGC |
| TDR | 87,797,084 | 87,797,105 | CCCACGAAGTAGAAGGCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | GCCGCGAGAGGCTGTAAGGC |
| SD | GCAGAGGCTGAACAAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
