11180 Rnase11 Status: ES cell colonies screened / QC no positives
| Gene | Rnase11 |
| Chromosome | 14 |
| Deletion Start | 51,050,092 |
| Deletion End | 51,049,472 |
| Deletion Size | 621 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,050,060 | 51,050,077 | TTGCTCGCCCTTGGACTG |
| TUP | 51,050,029 | 51,050,057 | TCTTGCAAAGCCTTCAGAAAGCAGAATGA |
| TUR | 51,049,983 | 51,050,002 | GCAGCAGGTTGCATCTCTTC |
| TDF | 51,049,680 | 51,049,701 | GTGAACAAACACCCAAGCCAAG |
| TDP | 51,049,646 | 51,049,669 | AGCCTAGGACTGGAGCGTACCACG |
| TDR | 51,049,606 | 51,049,624 | CATGGGACCTGAGGCACTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGGTCCCTGCACATTAG |
| SD | GGATCTGTTGATGCCGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
