11183 Rbm38 Status: ES cell clone microinjected
| Gene | Rbm38 |
| Chromosome | 2 |
| Deletion Start | 173,022,043 |
| Deletion End | 173,033,829 |
| Deletion Size | 11,787 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 173,022,855 | 173,022,872 | GGTCGCAAGGCCAATGTG |
| TUP | 173,022,874 | 173,022,895 | ACCTGGCCTACCTGGGTGCCAA |
| TUR | 173,022,934 | 173,022,955 | AGAAACAAGGAGGGCTGGAAAG |
| TDF | 173,033,314 | 173,033,333 | GCTAAGTGGCCTGTGGAGTG |
| TDP | 173,033,338 | 173,033,361 | CACTGTCTGTGCAAAGGCCCTGGG |
| TDR | 173,033,401 | 173,033,419 | CCAAGCCTGTGTACCCATC |
PCR Primers
| Primer | Sequence |
| SU | GGCTAAGTCACCTCATTCG |
| SD | CAAGCACGGCAGGGCTGCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 407 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 214 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
