11190 Usp13 Status: Cancelled
| Gene | Usp13 |
| Chromosome | 3 |
| Deletion Start | 32,903,076 |
| Deletion End | 32,933,230 |
| Deletion Size | 30,172 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,818,401 | 32,818,423 | CCATTTCCCATCCCATTCCTTTC |
| TUP | 32,818,428 | 32,818,450 | TATTGTCAGGTCCGGTGGTCCCA |
| TUR | 32,818,472 | 32,818,493 | CCATAATAAGCCAGCGGCAGAC |
| TDF | 32,847,047 | 32,847,066 | TCCCAGGACAAGCAAGTGAG |
| TDP | 32,847,067 | 32,847,094 | AAAGAAATCCCGAGTGTACAACGTGGCC |
| TDR | 32,847,114 | 32,847,133 | GGTCTGCCACAGGACCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCTGGTTCCCACTC |
| SD | ACCAACGGGACTGTAATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 85761 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 85285 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
