| Gene | Pnma5 |
| Chromosome | X |
| Deletion Start | 73,066,756 |
| Deletion End | 73,037,073 |
| Deletion Size | 29,684 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,036,412 | 73,036,432 | GCACTGTGGACTAGCAATGAC |
| TUP | 73,036,389 | 73,036,411 | TCCCTAACCGTGGAGCAGTGCTT |
| TUR | 73,036,315 | 73,036,337 | GAGAAGACTGAAGGAACCTGAAC |
| TDF | 73,035,984 | 73,036,001 | CACCCAGGCAGAGGATTC |
| TDP | 73,035,960 | 73,035,983 | TAGGGAGGTCAGCACCCAGACCAC |
| TDR | 73,035,923 | 73,035,940 | CAGTCGCCTCCGTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GTGTCCCTGGACTTGCAAG |
| SD | CCCTGGACAAAAGAGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 29582 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 30056 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11193A-D8 | VGB6 | C57BL/6NTac | |
| 11193B-F3 | VGB6 | C57BL/6NTac | |
| 11193B-G3 | VGB6 | C57BL/6NTac | |
