11195 Snapc2 Status: ES cell colonies screened / QC no positives
| Gene | Snapc2 |
| Chromosome | 8 |
| Deletion Start | 4,253,107 |
| Deletion End | 4,255,806 |
| Deletion Size | 2,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,253,825 | 4,253,842 | TTGGTAGCCCGGTGTGTG |
| TUP | 4,253,848 | 4,253,874 | CACCTCTAATCCTAGCACCTGGGATCC |
| TUR | 4,253,918 | 4,253,940 | GCATTTGCTGTCCTGGAACTATG |
| TDF | 4,255,462 | 4,255,486 | TCTCCTTTCCTTCTACCAGAGTCAG |
| TDP | 4,255,488 | 4,255,512 | TGTGGTCCTTAACCTGCTCATGGCA |
| TDR | 4,255,522 | 4,255,541 | CAAGGCAGGTGGGACAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCAGCTCACGAGTGAC |
| SD | CACAGTGGGCTAGAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
