| Gene | Cypt4 |
| Chromosome | 9 |
| Deletion Start | 24,625,219 |
| Deletion End | 24,627,200 |
| Deletion Size | 1,982 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,625,356 | 24,625,379 | TCGTTCTCATTCTCGTTCCTGTTG |
| TUP | 24,625,381 | 24,625,406 | TGCTGTCTTCGTTCTCGTAGCAGAAC |
| TUR | 24,625,463 | 24,625,486 | GAAGTTCAGTAGCACGCTTGATTC |
| TDF | 24,627,029 | 24,627,053 | CTCAGTCAGAGAAGATGCACCTAAC |
| TDP | 24,627,056 | 24,627,079 | TCAAGAGACTTGGAGCCTCAGGGA |
| TDR | 24,627,080 | 24,627,101 | ACACTCTACCAAACCTCCCTAC |
PCR Primers
| Primer | Sequence |
| SU | GGACATCTGAGATTCTCAGG |
| SD | ACATTGGCTTGTATGGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11198A-C3 | VGB6 | C57BL/6NTac | |
| 11198A-D4 | VGB6 | C57BL/6NTac | |
