11200 BC057022 Status: Cancelled
| Gene | BC057022 |
| Chromosome | 5 |
| Deletion Start | 114,594,389 |
| Deletion End | 114,612,193 |
| Deletion Size | 17,805 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,594,460 | 114,594,478 | GCGCAAGTGTGAGTGAGAC |
| TUP | 114,594,481 | 114,594,505 | TCCTGACGTGTTTCCTCTTCCTGCA |
| TUR | 114,594,518 | 114,594,538 | CAGACGCCCAAGACCAGAAAG |
| TDF | 114,611,965 | 114,611,986 | CCAGTCGCTGGAGTATCTCATC |
| TDP | 114,611,987 | 114,612,008 | AACGACATCCGGCCACCCTGCA |
| TDR | 114,612,033 | 114,612,051 | ACGGCCACCGTCTCGTAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGAGAGATGGAATGTCTG |
| SD | ATAGAGACCCCGTGTATGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
