11203 Slc7a12 Status: ES cell colonies screened / QC no positives
| Gene | Slc7a12 |
| Chromosome | 3 |
| Deletion Start | 14,480,800 |
| Deletion End | 14,505,586 |
| Deletion Size | 24,787 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGTCCAGGTTTGAGAATGCTTTG |
| TUP | | | CTGAACTTCCTAATGCC |
| TUR | | | GGACACTTGGAGAATGGCATCTG |
| TDF | | | GTGGGTTTCTGTGTTATTGGCTTC |
| TDP | | | AACCTTAATGGACATGCTA |
| TDR | | | TGGCAGTAGCAAGTGATTGTG |
PCR Primers
| Primer | Sequence |
| SU | ACAAGAAATCTGTTTGGTATGC |
| SD | TGGCAAAGTACATTTGCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| NeoFwd | SD | 420 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
