11206 Acss3 Status: Cancelled
| Gene | Acss3 |
| Chromosome | 10 |
| Deletion Start | 79,488,034 |
| Deletion End | 79,468,872 |
| Deletion Size | 304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,123,546 | 107,123,567 | TGCAATGTCGCAAAGTAACAGG |
| TUP | 107,123,506 | 107,123,528 | CACCTCTGCCAGGATCCCCTTCA |
| TUR | 107,123,459 | 107,123,477 | CCAAACCCGGCCACCAAAG |
| TDF | 107,123,408 | 107,123,429 | GCAGTGGTGGTGAGTACAAGAC |
| TDP | 107,123,386 | 107,123,407 | CCACTTCGCAGCCTCTGTGGCT |
| TDR | 107,123,355 | 107,123,375 | CAGCAGCTTTACCCCAGAATC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGTCAAATTGCAAGTG |
| SD | AGCCTTTGTTTTCCTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 27635965 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 27654224 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
