11212   Nat6     Status: ES cell colonies screened / QC no positives

GeneNat6
Chromosome9
Deletion Start107,582,911
Deletion End107,583,869
Deletion Size959
Genome Build38
MGI IDMGI:1888902
NCBI Gene ID56441
BAC ID418C3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,583,078107,583,097CCCAGAGATGAGCCTCAGTC
TUP107,583,098107,583,120CTGGCCCAGCTGAGCTTACCCTG
TUR107,583,127107,583,146AGCTCCTTTGCCTGGTGTTG
TDF107,583,549107,583,567GGGCTACCAACTGGGTGAG
TDP107,583,568107,583,591CCTGTGCAGGGTTTGGCCTTCACC
TDR107,583,601107,583,621ACGTAGGACAGTGGTGGACAG

PCR Primers

PrimerSequence
SUCTGTATCTTCCTGACACAGC
SDTCGGAAGTGCTCTGTGAGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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