11215 Spag11a Status: ES cell colonies screened / QC no positives
| Gene | Spag11a |
| Chromosome | 8 |
| Deletion Start | 19,157,948 |
| Deletion End | 19,159,413 |
| Deletion Size | 1,466 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,158,297 | 19,158,319 | GGTAGCTGTGGACAAAGGGTAAG |
| TUP | 19,158,321 | 19,158,344 | TTACTGCCACGCTGTGAGCAGAGA |
| TUR | 19,158,352 | 19,158,372 | AGGGAAGTGCCGTTAGATGTG |
| TDF | 19,159,263 | 19,159,282 | TCCCACCCGGAATCAGAAAC |
| TDP | 19,159,285 | 19,159,306 | CGTGTGCCTCATGCAACAGGGC |
| TDR | 19,159,316 | 19,159,336 | ACCAGAACGGCACATGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGAGAGTAGCTGGTACC |
| SD | GAGCTTGGTTTCTGCTTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
