11219 Cypt3 Status: Cancelled
| Gene | Cypt3 |
| Chromosome | X |
| Deletion Start | 153,558,616 |
| Deletion End | 153,559,301 |
| Deletion Size | 704 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,558,841 | 153,558,863 | TGTTATCGTTCTCGTCCTTCTCG |
| TUP | 153,558,874 | 153,558,893 | ACATTTAAGGTGAGAGATCC |
| TUR | 153,558,894 | 153,558,915 | GGGAGGGAATGAGCTTAGCTTG |
| TDF | 153,559,094 | 153,559,117 | GGAGACGAATCAAGAGTGCTACTG |
| TDP | 153,559,123 | 153,559,145 | CGATTGATGCGGAGCCAACTGGA |
| TDR | 153,559,153 | 153,559,174 | AAGCCATAGTCGGTTCCGGTTC |
PCR Primers
| Primer | Sequence |
| SU | ACTATACCTGGCTGGCATAG |
| SD | TCCTGTGTCTGCCGATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
