11220 Spata17 Status: ES cell colonies screened / QC no positives
| Gene | Spata17 |
| Chromosome | 1 |
| Deletion Start | 187,215,282 |
| Deletion End | 187,183,210 |
| Deletion Size | 32,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 187,214,890 | 187,214,909 | CAGGCAGGAGGATGACTGTG |
| TUP | 187,214,866 | 187,214,887 | TCCAGTCCAGCCTGGGCAACAC |
| TUR | 187,214,819 | 187,214,842 | TTTGAGGAAGACTCCCAAATGTTG |
| TDF | 187,183,773 | 187,183,797 | TGGAAGCACATAGCATCTCACATAG |
| TDP | 187,183,729 | 187,183,754 | TTTGCCTCATCACTGCATCCGTATCC |
| TDR | 187,183,704 | 187,183,726 | GCTGTCTTCAGTCGAAACCATTC |
PCR Primers
| Primer | Sequence |
| SU | CCAATTCCACACAGGACTTC |
| SD | CCTGACACTGTGAAGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
