11221 S100a7a Status: ES cell colonies screened / QC no positives
| Gene | S100a7a |
| Chromosome | 3 |
| Deletion Start | 90,655,511 |
| Deletion End | 90,657,845 |
| Deletion Size | 2,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,655,888 | 90,655,911 | CTCCAGTACCATGAAGAGCTAATC |
| TUP | 90,655,913 | 90,655,938 | CTGAGACTACAGCCTGACACCACACC |
| TUR | 90,655,943 | 90,655,964 | GGACACAGGAGGCAGAAGAATC |
| TDF | 90,657,043 | 90,657,063 | CTTCAGCCTCAGGGTTCTCAG |
| TDP | 90,657,069 | 90,657,094 | ATATCCTGGGCAGCTTCTGTGGCCAT |
| TDR | 90,657,097 | 90,657,117 | GGAAAGTGGACTAGGGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGTGCCTTGCTTCAAACC |
| SD | AGGGATACCCAGAACCATGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
