11226   Bcl2a1b     Status: ES cell colonies screened / QC no positives

GeneBcl2a1b
Chromosome9
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1278326
NCBI Gene ID12045
BAC ID295D8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCACTCCCTGGCTGAGCACTAT
TUPTCAGTATGTGCTACAGGTACCCGCCT
TURCTTTGTAGCACTCTGCATGCTTT
TDFATGCTTAGATATCAGGGAGTGGCAG
TDPTAGAAGGATTAAGAGGTGTGGCCTTGTTGG
TDRGGACTGGGCTTTTGGGAAAC

PCR Primers

PrimerSequence
SUGGAAGTGGTGTGTTGCTCTG
SDCTTCCAGGTCATCATCTGTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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