11227 Svs3b Status: Cancelled
| Gene | Svs3b |
| Chromosome | 2 |
| Deletion Start | 164,256,618 |
| Deletion End | 164,255,550 |
| Deletion Size | 1,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,256,266 | 164,256,293 | CCTCACCACTCATGTTTATTGGGAAAAG |
| TUP | 164,256,233 | 164,256,260 | TCCTCTATGGGCACAAAGAAGAACAGGA |
| TUR | 164,256,167 | 164,256,188 | GCATCAGCATCCTGGCCATATG |
| TDF | 164,256,037 | 164,256,062 | CCCAGTCACAAATAAAATCCCAAACC |
| TDP | 164,256,004 | 164,256,031 | AAAATCCTACGCAGCCCAACTGAAGTCC |
| TDR | 164,255,947 | 164,255,973 | GGATTTCAGCATGGTTTGTGACTTCAT |
PCR Primers
| Primer | Sequence |
| SU | GACACTGTGGACAAGAGCCC |
| SD | TCTGGTCTCCAGATGACAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
