11234 Prr18 Status: ES cell colonies screened / QC no positives
| Gene | Prr18 |
| Chromosome | 17 |
| Deletion Start | 8,341,017 |
| Deletion End | 8,341,942 |
| Deletion Size | 926 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,341,177 | 8,341,196 | GCGGCGAGAAAAAGAGGAGG |
| TUP | 8,341,197 | 8,341,219 | CCGCCCGAGATGCTGCTCTCCAG |
| TUR | 8,341,228 | 8,341,245 | TCTTCAAGGTGGCGGAGG |
| TDF | 8,341,641 | 8,341,658 | AACGCGCCTCTGGCTGTG |
| TDP | 8,341,660 | 8,341,679 | CTGTCAGCAGCCGCCCTCCC |
| TDR | 8,341,790 | 8,341,810 | TCCTCCTCGTACTCCTCATCA |
PCR Primers
| Primer | Sequence |
| SU | GTGCGATCCAGGCGACATCC |
| SD | GTTAGAAGGCATTTCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 442 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 170 bp | + | + | - |
| NeoFwd | SD | 408 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
