11237 Gykl1 Status: Cancelled
| Gene | Gykl1 |
| Chromosome | 18 |
| Deletion Start | 52,693,706 |
| Deletion End | 52,695,418 |
| Deletion Size | 1,713 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,694,453 | 52,694,472 | AAGTGGCGTGTTGGAAGGTG |
| TUP | 52,694,473 | 52,694,501 | TGCCAATATCTGGATGTCTGGGAGACCAG |
| TUR | 52,694,520 | 52,694,539 | CCGTCCTGAAGACACAGTTG |
| TDF | 52,694,944 | 52,694,965 | TGGATGCCATGAACAGTGATTG |
| TDP | 52,694,966 | 52,694,988 | TGGAATCCCGCTGACGCATTTGC |
| TDR | 52,694,991 | 52,695,011 | TGCGGTCATTCCTCCATCTAC |
PCR Primers
| Primer | Sequence |
| SU | GGATGTTGGAGAGCAACTGG |
| SD | GTGTTATGCAGAGGTACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 398 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
