11239 Rnase4 Status: ES cell colonies screened / QC no positives
| Gene | Rnase4 |
| Chromosome | 14 |
| Deletion Start | 51,104,824 |
| Deletion End | 51,105,268 |
| Deletion Size | 445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,104,936 | 51,104,955 | GACAGCATGTGGACCCTCAG |
| TUP | 51,104,957 | 51,104,984 | TGACAGGTGGCAATGACAACTACTGCAA |
| TUR | 51,105,006 | 51,105,025 | TGCACTGGACAGAAGTCATC |
| TDF | 51,105,056 | 51,105,077 | TCTGGAACATTCGTGGCATCTG |
| TDP | 51,105,081 | 51,105,108 | TACCACCAATATCCTGTGCAAGAACGGC |
| TDR | 51,105,124 | 51,105,145 | CCGTGACCTTCACTACACCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCACCCAGTCATGTTTGTTC |
| SD | TGGCAGAATTGGTCTCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
