11240 Ferd3l Status: Cancelled
| Gene | Ferd3l |
| Chromosome | 12 |
| Deletion Start | 33,928,474 |
| Deletion End | 33,929,006 |
| Deletion Size | 533 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,928,516 | 33,928,534 | GGATGCTACCGTGCTGAAC |
| TUP | 33,928,536 | 33,928,560 | TCGTAGCAGATCTCTCTCTGGCCTC |
| TUR | 33,928,574 | 33,928,593 | GGTGGGAACTCGCAGAGAAG |
| TDF | 33,928,898 | 33,928,920 | AAGAGACTGTCGAGGATCGAGAC |
| TDP | 33,928,921 | 33,928,943 | CCTCCGCTTGGCCATCGTCTACA |
| TDR | 33,928,969 | 33,928,988 | CCTCCTTTTCCTCCTTGCTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGAACTGCCCTGCAGAGG |
| SD | AGCAACTAATCATGCGAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 411 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
