11242   Abpe     Status: ES cell colonies screened / QC no positives

GeneAbpe
Chromosome7
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:3042579
NCBI Gene ID381970
BAC ID252M14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF31,302,79331,302,814ACTTCTTCTGCTGGCCTTGCTG
TUP31,302,81631,302,837TGACTGGAGAGCTGGGCTTCCA
TUR31,302,84731,302,864TGAGCGAGGGTCCCATTC
TDF31,304,41631,304,438GGTGATGTCTGCCATAACTGAGG
TDP31,304,39231,304,414CGTCTGTCCAGGCACATTGGCTC
TDR31,304,30931,304,329GGTCCTGCAAAACCACAATCC

PCR Primers

PrimerSequence
SUTACAAAGGTCAGCTCTAACTC
SDATTAGAATCAGCCTAAGAGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR72 bp++-
TDFTDR130 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image