11244 Prap1 Status: ES cell colonies screened / QC positives
| Gene | Prap1 |
| Chromosome | 7 |
| Deletion Start | 140,093,464 |
| Deletion End | 140,097,104 |
| Deletion Size | 3,641 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,093,856 | 140,093,875 | TGCAGCTCAGGGTTTACCTC |
| TUP | 140,093,880 | 140,093,903 | TGGCCTCCAACTATGCATGAGGGT |
| TUR | 140,093,961 | 140,093,982 | CAGCCTGGAATGTTGCTTCTTC |
| TDF | 140,096,821 | 140,096,840 | ACAGGAGGCTAGTGGGAAGG |
| TDP | 140,096,844 | 140,096,871 | TTGTGAGGGAGGCTATATCTACGTCTCC |
| TDR | 140,096,874 | 140,096,894 | CAGGTCATGGCATCTGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTGGCTGGAAGTCTAGTG |
| SD | AAGAGAATTGTTCACGAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 410 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
