11249   Kbtbd12     Status: ES cell clone microinjected

GeneKbtbd12
Chromosome6
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1918481
NCBI Gene ID74589
BAC ID316N7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF88,618,71488,618,735GAGAAATTCCCTTGCCACAGAC
TUP88,618,69288,618,713TGGTGCTCGCTGCCTTTAGTCC
TUR88,618,66488,618,685TCCACAGGTGAACATAGCCTTG
TDF88,618,03288,618,051CGCTATGGCATGGAGACCAC
TDP88,618,00688,618,031CAGCCTTCTGCTTTGCATTGGGAACA
TDR88,617,97388,617,992AGCTCCTGTGCCTTGATCTG

PCR Primers

PrimerSequence
SUCTTTGATACAAAGTAGTTGTCC
SDAGTATGGCCAAGGCCATGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR72 bp++-
TDFTDR79 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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