11251 Kcnk13 Status: ES cell colonies screened / QC positives
| Gene | Kcnk13 |
| Chromosome | 12 |
| Deletion Start | 99,965,931 |
| Deletion End | 99,966,256 |
| Deletion Size | 326 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,965,978 | 99,965,997 | GCCACCTGAACGAGGACAAC |
| TUP | 99,966,001 | 99,966,021 | CGCTTCCTGCTGCTCGCTGGG |
| TUR | 99,966,022 | 99,966,043 | CCAGCAGGTAGAGCAGGATGAG |
| TDF | 99,966,136 | 99,966,156 | CACAACCTGAGCCGTGAAGAG |
| TDP | 99,966,158 | 99,966,179 | TGCGAGGTTTCCTCCGCCACTA |
| TDR | 99,966,200 | 99,966,218 | ACGCTGTCCATGCGGATGC |
PCR Primers
| Primer | Sequence |
| SU | GGAGAACTCCAGAGAGGAC |
| SD | TGGCTAACAAAGAGCAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 325 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
