11252 Ier5 Status: Cancelled
| Gene | Ier5 |
| Chromosome | 1 |
| Deletion Start | 155,099,427 |
| Deletion End | 155,098,471 |
| Deletion Size | 957 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,099,389 | 155,099,408 | ACCGCATCGTCAGCATCTCC |
| TUP | 155,099,363 | 155,099,388 | CTGGGCAAGATCTACAACTCGCGGGT |
| TUR | 155,099,311 | 155,099,331 | CAGCACCAACGACACCAAGAG |
| TDF | 155,098,705 | 155,098,724 | ACGACACCGACGAGGAGATG |
| TDP | 155,098,678 | 155,098,703 | AGACCGGGAACGTGGCAAACCTCATC |
| TDR | 155,098,653 | 155,098,675 | CCGAGAAGCTAGACCCGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GTGATTGGCGTTCGAGGAG |
| SD | ACACGGACACACACAGATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
