11254 Pgk2 Status: ES cell colonies screened / QC no positives
| Gene | Pgk2 |
| Chromosome | 17 |
| Deletion Start | 40,208,532 |
| Deletion End | 40,207,270 |
| Deletion Size | 1,263 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,208,196 | 40,208,218 | AGCCTGTGCCAACCCAGATAATG |
| TUP | 40,208,165 | 40,208,192 | CTATCATCCTGCTGGAGAACCTGCGCTT |
| TUR | 40,208,137 | 40,208,160 | TTTACCCTTACCTTCTTCCTCCAC |
| TDF | 40,207,458 | 40,207,481 | CCAAAGCTCTCATGGATGAAGTTG |
| TDP | 40,207,432 | 40,207,456 | AAAGGCCACCTCCAATGGCTGTGTC |
| TDR | 40,207,394 | 40,207,413 | GCGCAGCAAGTAGCAGTATC |
PCR Primers
| Primer | Sequence |
| SU | GGTAATCTTATGGTTGGGTATGC |
| SD | TCTCACAGCCTTTACCCTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
