| Gene | Magea9-ps |
| Chromosome | X |
| Deletion Start | 72,869,370 |
| Deletion End | 72,870,101 |
| Deletion Size | 732 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,869,607 | 72,869,627 | GCCTCTGTCAACCAAATGGAG |
| TUP | 72,869,635 | 72,869,657 | AGCCCATGGTATTGCAGCACGCA |
| TUR | 72,869,711 | 72,869,729 | CTTGTTGGTCAGCGCCTTC |
| TDF | 72,869,797 | 72,869,816 | CTGAGGGTTTGAAGCTGGTG |
| TDP | 72,869,818 | 72,869,845 | TTGGCATTGATGTGATAGAAGTGGACCC |
| TDR | 72,869,855 | 72,869,873 | CAGGGCAATGGCATAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TAGCTGTGCTTTGAAGACAC |
| SD | TGAATGTGGTGCTACTAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11255A-A6 | VGB6 | C57BL/6NTac | |
| 11255A-E1 | VGB6 | C57BL/6NTac | |
| 11255A-F5 | VGB6 | C57BL/6NTac | |
